Dr Daniel Ballesteros Bargues

Early Career Research Fellow

Daniel Ballesteros Bargues

Comparative Plant and Fungal Biology


Comparative Seed Biology


Structural biology of seeds and fern spores in relation to longevity and desiccation and low temperature stress tolerance, particularly during cryopreservation.

Biography Plus

My research in the Comparative Seed Biology group aims to reveal the fundamental basis of desiccation and low temperature stress tolerance in seeds, particularly in relation to successful cryopreservation. In addition to studies on recalcitrant seeds (e.g. oaks), I am interested in the variation in longevity in plant propagules, from fern spores as a unicellular model to more complex systems. Often these studies involve making structural and biophysical determinations. I also carry out teaching and training activities for staff and supervise undergraduate, MSc and PhD students.

Qualifications and appointments Plus
  • BSc in Biology. University of Valencia. Spain, 2001.
  • Pedagogic aptitude course (CAP) (equivalent to MEd). University of Valencia, Spain. 2002
  • Advanced Studies Diploma (DEA) (equivalent to MSc). University of Valencia, Spain 2004.
  • PhD in botany and plant physiology. University of Valencia, Spain. 2008.
  • Research Associate, Botanical Garden of the University of Valencia-ICBiBE (Germplasm Bank). Valencia, Spain, 2003-2007
  • Research Biologist, Pharmacology Department, University of Valencia, Spain, 2007-2009.
  • Postdoctoral Research Associate, National Center for Genetic Resources Preservation, ARS, USDA. Fort Collins, CO, USA, 2009-2011.
  • Postdoctoral Fellow, University of KwaZulu-Natal, Durban, South Africa, 2012-2013.
  • Postdoctoral Fellow, Center for Conservation and Research of Endangered Wildlife (CREW). Cincinnati zoo and botanical garden, USA, 2013-2014.
  • Research Biologist in the EU-ENPI funded project ECOPLANTMED. Center Conservation Biodiversity (CCB), Univeristy of Cagliari, Italy, 2014-2015.
Selected publications Plus

Ballesteros, D., Hill, L.M., Lynch, R.T., Pritchard*, H.W. & Walters, C. (2019).

Longevity of Preserved Germplasm: The Temperature Dependency of Aging Reactions in Glassy Matrices of Dried Fern Spores.

Plant and Cell Physiology 60: 376–392.   

Ballesteros, D., Hill, L.M., Walters, C. (2017)

Variation of desiccation tolerance and longevity in fern spores.

Journal of Plant Physiology, 211: 53–62

Ballesteros, D., Pence, V.C. (2017)

Survival and death of seeds during liquid nitrogen storage: a case study on seeds with short lifespans.

Cryoletters 38(4): 278-289

Ballesteros, D., Sershen, Berjak, P., Vargheese, B., Pammenter, N.W. (2014)

Rapid drying of zygotic embryos and embryonic axes from recalcitrant seeds for cryopreservation: challenges and considerations.

Cryobiology, 69: 100-109

Walters, C., Ballesteros, D., Vertucci, V.A. (2010)

Structural mechanics of seed deterioration: Standing the test of time.

Plant Science 179: 565-573

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Daniel Ballesteros

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Related links

BBC One, Countryfile

Cincinnati Enquirer: Zoo pulls plants out of deep freeze