People
Nadarajan, Jayanthi, Dr
| Job Title | Low Temperature Seed Researcher |
|---|---|
| Department | Seed Conservation |
| Section | |
| Science Teams | |
| Joined Kew | 2006 |
| Foreign Language(s) | Tamil, Malaysian Language (Bahasa Malaysia) |
Qualifications & Appointments
BSc (Hons), Botany, University of Malaya, Kuala Lumpur, Malaysia, 1996
M.Agric.Sc, Seed Technology, University Putra Malaysia, Malaysia, 1999
PhD, University of Abertay Dundee. Dundee, Scotland.UK, 2005
Role
Researching low temperature seed storage conditions for different seed traits; developing cryopreservation protocol for non-orthodox seeds including embryo rescue technique.
Conducting comparative studies of seed longevity and understanding the effects of storage environment – in particular, temperature on seed longevity. This involves investigating optimal long-term storage temperatures other than seed bank temperature. This research comprise of a long term (10-30 years) storage experiment for selected species with different traits such as size, oil content, origin, dicotyledon/monocotyledon and moisture content. Develop cryopreservation protocol for intermediate and recalcitrant seeds which are sensitive to desiccation and low temperature hence not storable under seed bank condition. This includes investigating different cryopreservation techniques such as encapsulation-dehydration, vitrification and droplet-freezing.
Projects
Do ‘Intermediate’ Seeds Exist? Towards a Mechanistic Understanding of Cold Stress
Ex Situ Conservation of Recalcitrant Seeds: Towards the Delivery of Target 8 of the GSPC
Selected Publications 2001-2005
Nadarajan, J., Staines, H.J., Benson, E.E., Mansor, M., Krishnapillay, B. and Harding, K. (2006). Optimization of cryopreservation for Sterculia cordata Blume. zygotic embryo using Taguchi experiments. Journal of Tropical Forest Science 18(4):166-172.
Johnston, J., Dussert, S., Gale, S., Nadarajan, J., Harding, K. and Benson, E. (2006). Optimisation of the azinobis-3-ethyl-benzothiazoline-6-sulphonic acid radical scavenging assay for physiological studies of total antioxidant activity in woody plant germplasm. Plant Physiology and Biochemistry 44(4): 193-201
Muthusamy, J., Staines, H.J., Benson, E.E., Mansor, M., and Krishnapillay, B. (2005). Investigating the use of fractional replication and Taguchi techniques in cryopreservation: a case study using orthodox seeds of a tropical rainforest tree species. Biodiversity and Conservation 14: 3169-3185.
Villalobos, V.M. and Engelmann, F. (editors). IPGRI Technical Bulletin No. 7. pp 73-75.
Benson, E.E, Johnston, J., Muthusamy, J and Harding, K (2005) Physical and Engineering Perspectives of In Vitro Plant Cryopreservation pp 441-473 In: Plant Tissue Culture Engineering. Ed. S. Dutta Gupta, Y. Ibaraki. Springer,Netherlands.
Krishnapillay, B. Nadarajan, J. and Engelmann, F. (2002). Tropical rainforest trees. In: In vitro collecting techniques for germplasm conservation. Pence, V.C., Sandoval, J.A.,
Selected Publications pre-2001
Nadarajan, J. and Krishnapillay, B. (1998). Handling and storage of recalcitrant and semi-recalcitrant tropical forest seeds. The desiccation trials of Hopea odorata seeds. Published in IPGRI Newsletter No.3 1998.
Nadarajan, J., Y.L. Hor and B. Krishnapillay. (1998). A comparative study on the effects of moisture content and temperature on storability of a semi-recalcitrant and orthodox seeds. In: Proceedings of the IUFRO Seed Symposium 1998. ‘Recalcitrant Seeds’. 12-15 October 1998. Kuala Lumpur, Malaysia. pp 48-52
Nadarajan, J. and Krishnapillay, B. (1999). Handling and storage of recalcitrant and semi-recalcitrant tropical forest seeds. The desiccation trials of Shorea leprosula seeds. Published in IPGRI-DFSC Newsletter No.5.
Nadarajan, J. and Krishnapillay, B. (1999). Handling and storage of recalcitrant and semi-recalcitrant tropical forest seeds. The storage trials of Hopea odorata seeds. Published in IPGRI-DFSC Newsletter No.4.