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Prychid, Christina J.

Job title: 

Lab Manager, Imaging and Electron Microscopy

Department: 
Comparative Plant and Fungal Biology
Joined Kew: 

1992

Foreign languages: 

Basic French, basic Polish

Qualifications and appointments: 
  • BSc (Hons), Molecular Biophysics, University of Leeds
  • MSc, Crystallography, Universty of London (Birkbeck College).
Role: 

My role is to oversee and manage the imaging and electron microscopy facilities at Kew, to provide consultation and training for users, to keep up to date with current research and to develop and undertake collaborative projects with researchers both internal and external, using cell biology and microscopy techniques.

My expertise is in the application of imaging and microscopical techniques to help explain biological questions. For example, during recent PhD studies on phylogenetics and floral biology of Mapanioid sedges, I used laser capture microdissection microscopy to extract pure cell-line populations from wax-embedded sectioned plant material. These were used for downstream mRNA extraction, next-generation sequencing and transcriptome analysis, enabling accurate determination of gene expression in different reproductive organs at different stages of development. This would otherwise be impossible to do in tissues too small for dissection by hand.

Selected publications: 

Rudall P.J., Prychid C.J. & Gregory T. (2014). Epidermal patterning and silica phytoliths in grasses: an evolutionary history. Botanical Review DOI 10.1007/s12229-014-9133-3

Prychid C.J. & Bruhl J.J. (2013). Floral ontogeny and gene protein localisation rules out euanthial interpretation of reproductive units in Lepironia (Cyperaceae, Mapanioideae, Chrysitricheae). Annals of Botany 112: 161-177.

Brown S.L., Warwick N.W.M. & Prychid C.J. (2013). Does aridity influence the morphology, distribution and accumulation of calcium oxalate crystals in Acacia (Leguminosae: Mimosoideae)? Plant Physiology and Biochemistry 73 DOI 219e228

Prychid C.J., Sokoloff D.D., Remizowa M.V., Tuckett R.E., Yadav S.R. & Rudall P.J. (2011). Unique stigmatic hairs and pollen-tube growth within the stigmatic cell wall in the early-divergent angiosperm family Hydatellaceae. Annals of Botany 108: 599-608.

Prychid C.J., Jabaily P.S. & Rudall P.J. (2008). Cellular ultrastructure and crystal development in Amorphophallus (Araceae). Annals of Botany 101: 983-995.